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KMID : 0043319980210020217
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Archives of Pharmacal Research
1998 Volume.21 No. 2 p.217 ~ p.222
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Resolution of Salbutamol Enantiomers in Human Urine by Reversed-Phase High Performance Liquid Chromatography after Derivatization with 2,3,4,6-Tetra-O-acetyl--D-glucopyranosyl Isothiocyanate
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Kim Kyeong-Ho
Kim Tae-Kyun
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Abstract
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A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl--D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs=1.83) on octadecylsilan column using 35% acetonitrile in 0.05M ammonium acetate buffer (pH=6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N=3).
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KEYWORD
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Chiral separation, HPLC, Chiral derivatizing reagent, Salbutamol, Enantiomer, 2, 3, 4, 6-Tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate
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